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Oximetry Monitoring in Photodynamic Therapy for Dosimetry


 

Skin cancer treatment

Photosensitizers

Chick embryo (chorioallantoic membrane)

Rat prostate tumor cells


 
 

Background

During photodynamic treatment (PDT) of cancer, a photosensitive drug is introduced and activated by light to induce cytotoxicity. While PDT offers great promise as a targeted cancer treatment, many attempts to use PDT in the clinic have been hindered by the complex dosimetry problem and lack of an accepted definition of dose, along with the techniques to measure it in vivo. A comprehensive approach to dosimetry would enable clinicians to optimize treatments for individual patients. The measurement of oxygen concentration during PDT is a particularly challenging problem due to real-time changes in oxygen demand and supply during the treatment.

Solution

Using a unique combination of magnetic and optical modalities, INO has developed and patented a technology platform that enables monitoring of photoactive species produced in (or via) photodynamic therapy (eg. reactive oxygen species). Magnetic-induced changes in the steady-state, time-resolved, and spectrally resolved emission of the drug can then be correlated to the oxygen/hypoxic status relevant to the reaction dynamics during treatment, potentially allowing the physician to adapt treatment to the patient response near real-time.

 

Plus…

The magnetic field effects on optical properties of PDT sensitizers which combine both a “drug-probe” constituents have been demonstrated with custom designed agents, to extract and dynamically monitor the oxygenation and/or reactive oxygen species.

Additional information

Photodynamic Therapy

Publications

Noiseux, I., Mermut, O., Bouchard, J.-P., Cormier, J.-F., Desroches, P., Fortin, M., Gallant, P., Leclair, S., Vernon, M., Diamond K. R., Patterson, M.S. “Effect of liposomal confinement on photochemical properties of photosensitizers with varying hydrophilicity” J. Biomed. Opt., 13(4), 041313, 2008.

Mermut, O., Noiseux, I., Bouchard, J.-P., Cormier, J.-F., Desroches, P., Fortin, M., Gallant, P., Leclair, S., Vernon, M., Diamond K. R., Patterson MS. “Effect of liposomal confinement on photo-thermal and photo-oximetric fluorescence lifetimes of photosensitizers with varying hydrophilicity” J. Biomed. Opt., 13(4), 041314, 2008.

Mermut O, Diamond KR, Cormier JF, Gallant P, Hô N, Leclair S, Marois JS, Noiseux I, Morin JF, Patterson MS, Vernon ML. “The Use of Magnetic Field Effects on Photosensitizer Luminescence as a Novel Probe for Optical Monitoring of Oxygen in Photodynamic Therapy” Phys. Med. Biol., accepted 2008.

Mermut O, Noiseux I, Bouchard JP, Cormier JF, Desroches P, Fortin M, Gallant P, Leclair S, Vernon M, Diamond KR, Patterson MS. “Effect of liposomal confinement on photo-thermal and photo-oximetric fluorescence lifetimes of photosensitizers with varying hydrophilicity” Virtual Journal of Biological Physics Research 16(2) 2008.

Mermut, O., Bouchard, J.-P., Cormier, J.-F., Desroches, P., Fortin, M., Gallant, P., Leclair, S., Noiseux, I., Vernon, M., Diamond K.R., Patterson, M.S. "Use of Magnetic Fields to Probe and Alter Photodynamic Processes in Photosensitizers," in Biomedical Optics, Optical Society of America (OSA) Technical Digest, paper BWB4, 2008

Mermut, O., Bouchard, J.-P., Cormier, J.-F., Desroches, P., Diamond K. R., Fortin, M., Gallant, P., Leclair, S.; Marois J.S., Noiseux, I., Morin J.-F, Patterson M.S., Vernon, M., “Time-resolved luminescence measurements of the magnetic field effect on paramagnetic photosensitizers in photodynamic reactions”, in New Sensitizers, Proc. of SPIE, vol. 6845 - Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic, paper 68450T, 2008.

Mermut, O., Bouchard, J.-P., Cormier, J.-F., Diamond K. R., Noiseux, I., Vernon, M.L and Patterson M.S. "Frequency domain, time-resolved and spectroscopic investigations of photosensitizers encapsulated in liposomal phantoms," in Therapeutic Laser Applications and Laser-Tissue Interactions III, Proc. of SPIE Volume 6632 - Progress In Biomedical Optics And Imaging, (OSA) paper 663218, 2007.

Mermut, O., Bouchard, J.-P., Cormier, J.-F., Diamond, K.R., Gallant, P., Noiseux, I., Vernon, M., Patterson M.S. “Frequency domain, time-resolved and spectroscopic investigations of photosensitizing drugs encapsulated in liposomal phantoms” in Biophotonics 2007, Vol. 3, 21, 2007.

Conferences

Mermut, O. “The effect of magnetic fields on photodynamic processes in cancer phototherapy” Gordon Research Conference - Lasers in Medicine and Biology, Holderness, NH, USA, July 20-25 2008.

Mermut, O. “Biophotonics Research and Innovation” Canadian Organization of Medical Physicists Annual Meeting, Quebec City, Canada, June 25-28 2008.

Mermut, O.“Time-resolved luminescence measurements of the magnetic field effect on paramagnetic photosensitizers in photodynamic reactions”, Engineering Conferences International: Biochemical Engineering XV. Controlled liposomal systems for photodynamic therapy and pharmaceutical applications, Quebec City, Canada, July 15-19 2007

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